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Reproducing pediatric kidney disease from human iPS cells

Scientists in Japan have found a skeleton key for congenital kidney disease research. Using iPS cells generated from the skin cells of a patient with a nephrin mutation, Kumamoto University scientists have successfully developed kidney tissue that exhibits the early stages of congenital kidney disease. The protein nephrin is a constituent of the kidney filtration membrane and abnormalities of this protein are commonly found in other types of kidney diseases. The results of this work are expected to unlock several doors in future kidney disease research.
The kidneys are organs that filter out and discharge waste products from the blood. During this process, proteins in the blood should not leak into the urine. The membrane responsible for this filtration is part of the glomerular podocyte and nephrin is its main constituent. If there is a genetic mutation affecting the nephrin, a large amount of protein in the blood can leak into the urine resulting in congenital nephrotic syndrome. The treatment of this syndrome is radical and difficult, and the absence of a technique to artificially reproduce a mutated filtration membrane has been a bottleneck in research progress.
In 2014, this Kumamoto University research group succeeded in the extremely difficult task of producing artificial kidney tissue in vitro from human iPS cells--a world first. And in 2016, they found that glomerular podocytes derived from iPS cells strongly express nephrin, that the human glomeruli readily connects with mouse blood vessels, and that induced podocytes continue to mature after transplantation into mice. With this knowledge, they applied their techniques to iPS cells derived from a single patient for their most recent project.
First, they established iPS cells from the skin cells of a patient with congenital nephrotic syndrome having a single nephrin mutation. When they induced kidney tissue from these iPS cells in vitro, they found that nephrin, which should exist on the surface of the glomerular podocyte, remained inside the cell, and that almost no filtration membrane precursor could develop. When podocyte maturation was performed by transplantation into mice, nephrin, which typically moves toward the blood vessel side of normal podocytes again remained inside the patient-derived cells. In other words, the initial pathology of this congenital kidney disease was reproduced by the iPS cells derived from the diseased patient.
Furthermore, when the nephrin mutation in patient-derived iPS cells were repaired and then induced into kidney tissue, the abnormalities were normalized. Put simply, the researchers found that this single mutation is the cause of the disease, and showed that it could possibly be treated by repairing the mutation.
"Because we can now reproduce the pathology of congenital nephrotic syndrome, it should be much easier to explore therapeutic drugs using this podocyte," said project leader Professor Ryuichi Nishinakamura. "Even when kidney disease develops in adults, it often begins with abnormal quantities of protein in the urine. It is believed that this is often caused by trouble in the nephrin protein of the filtration membrane. If a medication is discovered that controls nephrin protein, there is a strong possibility that it will be highly effective for kidney disease. What we have achieved here is a big step towards the development of drugs that act on podocytes and reduce proteinuria."
This research result was posted online in "Stem Cell Reports" on 30th Aug 2018.
*This research was a collaboration between Kumamoto University, Juntendo University, University of Ryukyus, and Hiroshima University.

Tanigawa, S. et al., 2018. Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes. Stem Cell Reports, 11(3), pp.727–740. Available at: http://dx.doi.org/10.1016/j.stemcr.2018.08.003.
[Paper Info]
Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes
Shunsuke Tanigawa, Mazharul Islam, Sazia Sharmin, Hidekazu Naganuma, Yasuhiro Yoshimura, Fahim Haque, Takumi Era, Hitoshi Nakazato, Koichi Nakanishi, Tetsushi Sakuma, Takashi Yamamoto, Hidetake Kurihara, Atsuhiro Taguchi, and Ryuichi Nishinakamura
Stem Cell Reports
Aug 30, 2018
(1) Japan Society for the Promotion of Science (KAKENHI), (2) A grant from the Research Center Network for Realization of Regenerative Medicine - The Japan Agency for Medical Research and Development (AMED), (3) The Takeda Science Foundation Grant
(1) JP17H06177, (2) NA, (3) NA


Glomerular podocytes and filtration membranes
A: Structure of glomerulus
B: Magnified picture of podocytes showing many protrusions
C: Schematic diagram of the filtration membrane between the protrusions of podocytes. Nephrin, the main component of the filtration membrane, works by forming a sieve.


Abnormal podocytes generated from patient-derived iPS cells
Kidney tissue was derived from iPS cells by inducing kidney progenitor cells (original cells) with two methods, induction in vitro (left) or transplantation into mouse kidney tissue (right). The process took a total of about 1 month (13 days + 20 days).
CREDIT: Prof. Ryuichi Nishinakamura
USAGE RESTRICTIONS: <p>Tanigawa, S. et al., 2018. Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes. Stem Cell Reports, 11(3), pp.727–740. Available at: http://dx.doi.org/10.1016/j.stemcr.2018.08.003.</p>
<p>This image is made available under the terms of the <a href=" https://creativecommons.org/licenses/by-nc-nd/4.0/" target="_blank"> Attribution-NonCommercial-NoDerivatives 4.0 International</a>. Any further distribution of this work must follow the terms of this license. </p>


Filtration membrane precursors are reduced in patient-derived podocytes
A: Glomerulus and podocyte induced in vitro
B: Podocyte after mouse transplantation
Filtration membrane precursors (white arrows), which normally exist on the sides and bottom of podocytes, are rarely detected in patients.
Scale bar: 10 nanometers
[Images Details]
CREDIT: Prof. Ryuichi Nishinakamura
USAGE RESTRICTIONS: This image may only be used in conjunction with the accompanying release, or stories written about the work described in the release with reference to the original work.